TNAU Agritech Portal
Home | About Us | Success Stories | Farmers Association | Farmers' Innovation | Publications | Photo Bank | Contact
Crop Improvement :: Emasculation and Pollination Techniques

EMASCULATION AND POLLINATION TECHNIQUES OIL SEEDS

GROUNDNUT (Arachis hypogaea) (2n = 40)Family – Fabaceae

Self pollination is the rule in groundnut. Anthesis commences at 6 am and continues upto 8 am.  Anther dehisces two hours prior to opening of the flower.  Twenty four hours  before anthesis, the buds are very small. During the day, elongation of calyx, proceeds slowly but process gets accelerated during night.

Selfing

Since cleistogamous condition prevails in groundnut, selfing is most easy in this crop.  Usually covering is unnecessary and difficult.  Keeping the plants in insect proof cages will ensure self pollination.

Crossing

Mature flower buds which are ready to open in the next day are selected and emasculated in the evening.  They can be easily identified by the size and length of calyx tube.  The flower bud of groundnut is of crescent shape, being bulged on one side and slightly depressed on the other.  The keel petal is located on the bulged side and the standard is present on the depressed side.  For emasculation, hold the bud between the thumb and the index finger of the left hand and with the help of a razor blade in the right hand, make a cut on the depressed side at two-thirds the length below the tip so as to cut the standard and a portion of the wing petals.  Then gently pull the calyx and corolla by holding at the tip of the flower bud.  By doing this, the sepals and the petals except the keel would be removed, with the help of the fine forceps gently liberate the bundle of stamens and the pistil from the keel and nip off the anthers.

With a hand lens, examine the tips of filaments so as to ensure complete removal of the anthers.  Take a piece of straw tube (used for sipping cool drinks), 4 to 5 cm long and close one side opening by bending the tip.  Cover the emasculated flower bud with the straw tube by slowly inserting calyx tube into it.  This would ensure perfect protection to the stigma from any natural cross pollination.  The next morning take out the straw tube, dust the stigma with the desired pollen and reinsert the tube.  Pollination between 7 and 8 am was found to give more success.  If the stigma is found dry, pollinate after smearing it with 2 per cent sucrose solution.

Next day early morning between 7 am and 11 am pollen is collected from mature yellow anthers of the selected male parent and dusted on the receptive stigma.  For cross pollination, the selected male flower is held between thumb and the middle finger after the standard and wing petals are removed.  The flower with keel protruding is taken to the stigma of the emasculated flower.  A gentle push on gently keel by the finger forces lumps of pollen grains of the cover the entire stigmatic surface.  Five to seven days after pollination successful crosses will produce gynophores (pegs) with the dried flowers at their tips.  These are then introduced into small wire rings of 4 mm diameter which are marked for respective crosses.

SESAME (Sesamum indicum) (2n = 26)Family – Pedaliaceae

Gingelly is a self pollinated (Autogamous) crop. In some varieties cross pollination also takes place to a limited extend of 5-6 per cent. Very high cross pollination between 14 and 65 per cent has been recorded in a few varieties in India. Hence, the crop can be classified as often cross pollinated. Cross pollination may occur due to wind and bee activities. On a bright clear day, the flowers open between 5 and 7 am.  In the mature flower bud, just before the flower opens, the four unripe anthers are much below the stigma which at this stage is not receptive.  The anthers begin to burst longitudinally after 4am in the next day and commence to liberate their pollen.  At this time, the stigma becomes receptive.  The plant comes to flowering 4 weeks after sowing. 

Selfing 

  1. Tieing with thread: Selfing can be effected by tieing the corolla of the unopened flower which is selected in the previous day evening itself.
  2. Smearing of semi-solid clay: Selfing can be done by smearing a speck of semi-solid clay, on the upper portion of tubular petals of unopened flowers.  The clay while on drying does not allow the tubular petals to open and hence self pollination is the rule. This method is cheap and less time consuming one.   This method is noat effective during rainy days.  During rainy days, fevicol may be applied on young flower bud to ensure selfing.

Crossing

Soda- straw method

The emasculation technique in sesame is easy for crossing due to epipetalous nature of the stamens. The flower bud which is expected to open in the next day morning is selected in the previous day evening between 3 P.M. and 6 P.M. and emasculated by just removing the corolla tube in which the stamens are attached. Then, the emasculated flower buds are covered with a piece of soda-straw tube, bent at the top in order to avoid contamination from foreign pollens. During the next day morning, between 7 A.M. and 9.A.M., pollen from the desired male parents were dusted gently on the surface of the stigmas of the emasculated flower buds after removing the soda-straw and again covered.  The unemasculated flowers are removed in the female parent.

Individual crossed flowers are tagged with coloured thread for the identification of crossed capsules.  Different coloured threads are used for different type of crosses.

Content on this page requires a newer version of Adobe Flash Player.

Get Adobe Flash player

Sesamum (selfing and Crossing Techniques) - Video Clip (Duration: 01:10min)

SUNFLOWER (Helianthus annuus) (2n = 20)Family - Astraceae

Sunflower in highly cross pollinated crop mainly through insects (Entomophily) and to a limited extent by wind (Anemophily).  The flower opening starts from outerside of the head and proceeds towards centre.  The head takes 5-10 days for complete blooming depend on size of head and season.  Anthesis occurs between 5 to 8 A.M.  Pollen viable for 12 hours.  Stigma is receptive for 2-3 days.  The staminal filaments elongate rapidly and the anthers appear above the top of the corolla.  Anthers dehisce early than maturity of stigma. (Protoandry). In this crop, the cross pollination occurs due to protandry, limited area of stigmatic surfaces for receptivity, ray floret colour attracts insects and abundance of sweet secretions in the disc florets

Selfing 

The flower head is protected with a suitable cover before the commencement of anthesis in any of the florets and the cover is retained till fertilization is over in all the florets. Artificial self pollination with pollen collected from the same flower or another flower of the same plant using a soft brush will enhance seed settings.

Crossing

i. Hand Emasculation

Emasculation is done in the early morning by removing the anthers of the disc florets in 2 to 3 whorls with forceps and the other florets in the head are removed.  About 9-10 am the pollens from desired male parent are collected and dusted on the emasculated head.  This process is continued for 2 to 3 days.

ii. Without emasculation

In sunflower, head emasculation is difficult.  Considering this difficulty, the heads are pollinated without emasculation.  On the basis of hybrid vigour, plants are distinguished from the selfed plants.  The presence of marker genes for identifying hybrids is also utilised effectively.

iii. Chemical induction of male sterility.

The is achieved by spraying of 100ppm GA (Gametocide) during bud-initiation stage consecutively for three days in the morning.

Pollination is carried out by collecting pollen from heads which are already bagged prior to flowering. Pollen may be collected from flowering heads into paper bags. Pollination is done in the morning by applying the freshly collected pollen by a small piece of cotton, a hair brush or through fine cloth bag. After each cross, care must be taken to avoid contamination by wiping the hands with alcohol.

Content on this page requires a newer version of Adobe Flash Player.

Get Adobe Flash player

Sunflower (Selfing and Crossing Techniques) - Video Clip (Duration: 02:41 min)

CASTOR (Ricinus communis) (2n = 20)Family – Euphorbiaceae

Cross pollination is the rule in this crop.  It is mainly wind pollinated. But insect activity is also seen to some extend since the young leaves just below the inflorescence exude copious nectar at the time of flower opening cause insect pollination. Unisexual flowers, protogynous, elevated position of female flower in the inflorescence, mechanisms to promote wind pollination and nectar glands to attract insect promotes cross pollination. Here male flowers open first (protoandry).  After one or two days of male flowers opening, female flowers open.  However, protogyny is also reported.  The opening is between 4.30 and 5.00 A.M.  Pollen grains are viable for a 2 days and stigma is receptive for 3 days.  Each candle takes 10-12 days to complete flowering.

Selfing

The whole inflorescence is protected with not yet opened are selected. From the selected inflorescence all the male flowers are removed and the female flowers protected with a suitable cover. Artificial cross pollination is done when the stigmas of the retained female flowers become receptive by rubbing the anthers of male flowers collected from the selected male parent. During the rain day old bags are to be replaced with new bags to avoid fungal attack, and free air movement.

Crossing

  1. Emasculation: It can be achieved by removing or rubbing off the staminate flowers by finger and thumb.
  2. Crossing: Pollen grains are collected from the desired male parent and are dusted on the stigma of the female parent.  Again the inflorescence is covered.

Content on this page requires a newer version of Adobe Flash Player.

Get Adobe Flash player

Castor (Selfing and crossing Techniques) - Video Clip (Duration: 02:30min)

 
Home | About Us | Success Stories | Farmers Association | Publications | Site Map | Disclaimer | Contact Us

© 2015 TNAU. All Rights Reserved.