Farm Enterprises :: Mushroom

PREPARATION OF BED SPAWN

Bed spawns:

The method of preparation of bed spawn was same as that of mother spawn. The cooking, filling and sterilization were similar to that of mother spawn. Instead of saline bottles, polypropylene bags were used. After sterilization, the bags are taken for inoculation.

Procedure:

The sterilized bags are placed inside the culture room and put on the UV light.

After 20 minute put off the UV light and take the well-grown mother spawn.
Transfer spawn from the mother spawn to sterilized bags @ 10 g per bag.
After inoculation the spawn bags are kept in a clean room for fungal growth. (This is first generation of bed spawn).
Use the bed spawn after 10 days of inoculation for bed preparation.

Spawn preparation


White sorghum grains	Boiling (30min)	Shade drying	Mixing of CaCo3 20g/kg	PVC Rings

Preparation of Spawn bags


Step - 1	Step -2	Step- 3	Filling of bags with the grains

Hold the PVC rings	Plug with non absorbent cotton	Cover with paper and tied with the thread	Store in trays

Dettol, ethanol, inoculation needle, forceps, spatula, desection needle, match box, kinflim, blade	Laminar air flow chamber	Inoculation of bed spawn	Mother spawn

Bed spawn