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Bacterial leaf blight
(Xanthomonas oryzae pv. Oryzae)



Symptoms  
Seedling wilt or kresek
  • Observed 1-3 weeks after transplanting
  • Green water-soaked layer along the cut portion or leaf tip of leaves as early symptom
  • Leaves wilt and roll up and become grayish green to yellow
  • Entire plant wilt completely

Leaf blight
  • Water-soaked to yellowish stripes on leaf blades or starting at leaf tips with a wavy margin
  • Leaves with undulated yellowish white or golden yellow marginal necrosis, drying of leaves back from tip and curling, leaving mid rib intact are the major symptoms.
  • Appearance of bacterial ooze that looks like a milky or opaque dewdrop on young lesions early in the morning
  • Severely infected leaves tend to dry quickly
  • loss in grain yield may be up to 60%.

Confirmation
  • To distinguish kresek symptoms from stem borer damage, the lower end of the infected seedling can be squeezed between the fingers.
  • Yellowish bacterial ooze may be seen coming out of the cut ends.
  • The cut portion can be observed against the light to see the bacterial ooze streaming out from the cut ends into the water.
  •  After 1-2 hours, the water becomes turbid.
   
Seedling wilt-Kresek Leaves with wavy yellow
marginal necrosis
Drying and curling of
leaves leaving midrib
intact
Affected leaves with wavy
yellow marginal necrosis
   

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  Identification of pathogen
  • The bacteria causing the disease are rods, 1.2 x 0.3-0.5 µm. They are single, occasionally in pairs but not in chains.
  •  They are Gram negative, non-spore-forming, and devoid of capsules.
  •  Their colonies on nutrient agar are pale yellow, circular, and smooth with an entire margin. They are convex and viscid.


Factors favouring disease development

  • presence of weeds
  • presence of rice stubbles and ratoons of infected plants
  • presence of bacteria in the rice paddy and irrigation canals
  • warm temperature(25-30° C), , high humidity, rain and deep water.
  • Severe winds, which cause wounds, and over fertilization are suitable factors for the development of the disease.
  • Irrigation water and splashing or windblown rain can disseminate the bacterium from plant to plant.
  • The use of trimming tools for transplanting and by handling during transplanting can also trigger new infection.
Bacterial Ooze Microscopic View of
Xanthomonas Oryzae
Xanthomonas Oryzae

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Management Strategies  
Preventive method
  • Seed treatment with bleaching powder (100g/l) and zinc sulfate (2%) reduce bacterial blight.
  • Seed treatment - seed soaking for 8 hours in Agrimycin (0.025%) and wettable ceresan (0.05%) followed by hot water treatment for 30 min at 52-54oC;
  •  seed soaking for 8 hours in ceresan (0.1%) and treat with Streptocyclin (3g in 1 litre);
  • Spray neem oil 3% or NSKE 5%
  • Spray fresh cowdung extract for the control of bacterial blight. Dissolve 20 g cowdung in one litre of water; allow to settle and sieve. Use supernatant liquid. (starting from initial appearance of the disease and another at fortnightly interval)

Soak the Seeds in
Agrimycin for 8 hours
Spray Fresh Cowdung
Extract
  Spray Neem Oil Treat the Seeds with
Bleaching Powder


Cultural methods
  • Grow Tolerant varieties (IR 20 IR 72,PONMANI , TKM 6).
  • Secure disease free seed
  • Grow nurseries preferably in isolated upland conditions
  • Avoid clipping of seedlings during transplanting.
  • Balanced fertilization, avoid excess N - application
  • Skip N - application at booting (if disease is moderate)
  • Drain the field (except at flowering stage of the crop)
  • Destruction of weeds and  collateral hosts
  • Avoid flow of water from affected fields
  •  Maintain proper plant spacing
   
Avoid Field to Field
Irrigation
Maintain Proper Spacing


Chemical methods
  • Seed treatment with bleaching powder (100g/l) and zinc sulfate (2%) reduce bacterial blight.
  • Seed treatment - seed soaking for 8 hours in Agrimycin (0.025%) and wettable ceresan (0.05%) followed by hot water treatment for 30 min at 52-54oC;
  •  seed soaking for 8 hours in ceresan (0.1%) and treat with Streptocyclin (3g in 1 litre);
  • Spray Streptomycin sulphate + Tetracycline combination 300 g + Copper oxychloride 1.25kg/ha. If necessary repeat 15 days later.
  • Application of bleaching powder @ 5 kg/ha in the irrigation water is recommended in the kresek stage.
  •  Foliar spray with copper fungicides alternatively with Strepto-cyclin (250 ppm) to check secondary spread.
   
Foliar Spraying of Copper Fungicides Seed Treatment with Zinc Sulphate
 
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